How to isolate RNA from plants with high secondary metabolite production?

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Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

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How does grain and grain boundary affect the ceramic when studying its dielectric properties?

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What are the factors that causes the sample to have higher efficacy at lower concentration?

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31 July 2024 8,666 3 View

What topic or subject does Production Engineering need to address more?

Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?

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Why knockouts for same genes from two different gRNA are showing different result?

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Reason for discontinuities in my Band structure?

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If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

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How to dispose off lipids waste?

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What publications should I target as a psychology masters student in the UK?

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Is there a problem with my RNA pellet?

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Strugglling with m6A dot blot any suugesstion ?

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RNA Extraction Using Hot Borate Method No Longer Working?

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

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E.coli contamination in human RNA seq data ?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

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Weak DAPI staining after immunohistochemistry - how to improve?

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The Curse of Evolution and Complexity?

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Arvind Singh

Please check the following link.

http://googleweblight.com/?lite_url=http://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-4-85&ei=zxnZfJw3&lc=en-IN&s=1&m=708&host=www.google.co.in&ts=1484679414&sig=AF9NedkJ2aciMRIJUNF1tHe9q4BhEE7URQ

Please have a look at this link.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829573/

Preeti Sharma

Thank you Arvind, I have already tried with trizol and CTAB-LiCl method but the problem is with the integrity. I am getting very low RIN value and there is contamination of gDNA also.