Im looking for some answers about UV irradiation. I need to irridiate tk6 cell line to create pyrimidine dimers/ photo products damage (substrate DNA) to test the protein extract from solid tumour biopsies for NER activity. Suggest the protocol, which lamp to be used/purchased, how much dose, duration, and time or some other alternate strategy for the same.
Looking forward for ur reply. Kindly help me in this matter.
For our studies, we used UV crosslinker from Stratagene (see link below). Practically, all cell lines (or primary cells) that are to be exposed to UV for crosslinking require some optimization for exposure. Most details for exposure of cells are provided in the manual of the crosslinker.
https://ipmb.sinica.edu.tw/microarray/index.files/Stratalinker%20UV%20Crosslinker.pdf