I am using "pHrodo™ Red E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry" to access phagocytosis in neutrophils on perepheral whole blood.
As per the protocol i followed all the steps:
tube 1: Blood on Ice
tube 2: Blood+ beads on ice
Tube 3: Blood on 37 degree
Tube 4: Blood + beads on 37 degree
The tubes on ice are negative control for phagocytosis and activity is expected to be null.
I found 0% positivity in tube 1, tube 2 and tube 3, while 89% in tube 4 in a control.
Medians of tube 1 = 788, tube 2= 800, tube 3 = 750 and tube 4 is 1800.
How to interprete this data and report it.
Thanks in advance.
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