Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

In which solvent dissolve silver nanoparticles for XRD pattern study through NMR ?

The silver nanoparticles, which are solid form and I want to study the XRD pattern of this nanoparticles by Nuclear Magnetic Resonance (NMR), so I asked which solvent are used for dissolve of...

13 January 2021 3,434 4 View

Buckling of Smart composite plates using FEM ( MATLAB code)?

Can anyone help me with the FEM coding for Buckling of composite plates using the FSDT or HSDT - Source or the helping material (textbook, research paper etc) - Geometric stiffness matrix...

03 January 2021 3,059 3 View

How does one calculate interfacial heat transfer by using data from Fluent simulation for a subcooled flow boiling model?

I am trying to simulate subcooled boiling flow through an annulus using Fluent. Is it possible to get the values of interfacial heat transfer coefficients from either FLUENT report or CFD post?...

31 October 2020 3,599 3 View

Can someone help me with different watershed management practices for hilly areas ? Requirement is to have enough water for crop cultivation?

Can someone help me with different watershed management practices for hilly areas ? Requirement is to have enough water for crop cultivation. I am looking at the hilly areas, any proof of concept...

05 October 2020 5,641 15 View

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Due to COVID- 19, pupil teacher of teacher training programs are doing school internship in online mode which they are completely opposed to. What do you think as to it will adversely affect their...

09 August 2020 756 20 View

How best to get Cloud service credits from Cloud providers for my research simulations?

I need to run simulations running 100s of nodes in a simulated network and have to do that many times over. I could use Google Cloud Platform's $1000 provided for researchers doing PhD but I...

07 August 2020 4,537 3 View

Why is that only vaccine is available for Hepatitis B and not a drug that can eradicate it?

Hi Everyone, Entecavir 0.5 mg is usually given for patients with chronic hepatitis B to keep the virus under control and as well Vaccine is available for restricting the virus. If Entecavir 0.5...

22 June 2020 5,661 3 View

RT-PCR or Immunoassay? Or a combination?

So far, RT-PCR testing has been the frontline response to the COVID-19. PCR testing is considered as the gold standard for detecting COVID-19 and was designed by WHO, soon after the virus was...

21 April 2020 1,139 16 View

Is a combo of chloroquine and Zinc a cure for coronavirus?

Recent studies from Oklahoma Medical Research Foundation, University of Queensland Centre for Clinical Research, and Qingdao Municipal Hospital identified that Chloroquine phosphate, a drug used...

17 March 2020 6,929 94 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

No title

02 March 2021 3,060 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View