How to inhibit the synthesis of acetic acid in bacteria of the genus Acetobacter?

More Evgeniy Kiselev's questions See All

3D printing protein molecule models: FDM or SLA?

Hello I would like to 3D print models of protein molecules, and am thinking of buying a 3d printer. My question is – which technology would be better for such prints: FDM or SLA? To be more...

26 December 2022 2,456 4 View

Please recommend documents or research results on the development of electric water transport on European inland waterways ?

Dear Colleagues! Please recommend documents or research results on the development of electric water transport on European inland waterways

08 June 2021 6,399 2 View

ADP-Glo test for ATPase activity of NLRP3?

Hello colleagues! I want to investigate the atphase activity of nlrp3 in the ADP-glo test. but I don't know what type of buffer to use for analysis. Can anyone help me?

12 September 2020 7,793 3 View

Best tools for analyzing ptoein sequences?

Hi, everyone. So I’ve been assigned the task of evaluating effects that certain SNPs would have on some viral proteins. I’m a complete noob in bioinformatics, so where should I begin? Basically,...

27 April 2020 2,188 3 View

Building a PC for bioinformatics (mainly molecular dynamics)?

I would like to ask for some advice for building a personal computer for general home use and bioinformatics (mainly molecular dynamics, like CUDA calculations in GROMACS, AMBER, ect) Budget is...

18 April 2020 4,181 0 View

We are looking for a relation between Cartesian octupole and Mie scattering coefficients. Does anyone know the papers having an exact formula?

Dear Colleagues, We are looking for an accurate relation between the magnitude of Cartesian octupole and Mie scattering coefficients. Something very similar to formulas (8) and (9) from...

19 February 2020 4,027 4 View

How can i read VASP CHGCAR file?

Hello everyone. i was wondering could somebody help me with reading VASP CHGCAR files. I know i can use VESTA to plot them, but i'm interested in reading them by myself. So i have CHGCAR with...

09 November 2016 6,300 2 View

Does there exist the decomposition of a real 3rd order tensor?

Let T_{ijk} be a real 3rd order n x n x n tensor. Do there exist matrices U, V, W \in SO(n) such that the tensor S_{pqr} = sum_{i, j, k} U_{ip}V_{jq}W_{kr}T_{ijk} has the property: 1) S_{1qr} is...

27 June 2016 4,463 2 View

ALGO value infulence on the results. Why energies change?

Hello! I'm trying to calculate some band structures with HSE06 in VASP 5.2.12. No magnetism, with SOC, just bulk material. 8 electrons. FCC lattice.When i use ALGO = Damped, TIME = 0.4 and ENCUT =...

04 May 2016 8,829 9 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Reginald D. Smith

Well there are a couple ways if you mean chemical and not temperature. The first is to restrict the availability of ethanol and add acetic acid in moderate amounts to the medium. Without the presence of ethanol it will typically either metabolize acetic acid to CO2 and water via the citric acid cycle (overoxidation) or metabolize sugars to other organic acids like gluconic acid. If acetic acid is the only carbon source more acetic acid is not produced.

Evgeniy Kiselev

It is interesting to shift the metabolism towards the synthesis of polysaccharides (cellulose), but the resulting acetic acid inhibits this process. Processing of sugar into carbonic acid is not too interesting.