Use copper CuSO4. Copper induces the oxidation of lipoproteins by creating conjugated dienes.

All best

Dear Jumana Saleh

Is there is any specific protocol for oxidation of Lipoproteins? .

http://www.abd.or.kr/htm/kim/1999-11.pdf

this is one link. But you need to isolate LDL first.

All the best

Dear Jumana saleh

Thanks for your link.

Yes I already isolated LDL from plasma .

Oxidation can be carried ou by adding 2.5 µmol/L CuSO4 to human LDL (0.1 mg/mL of protein) and incubating for 2h at 37ºC. Oxidation kinetics may be monitored by continuously following the formation of conjugated diene, a product of lipid peroxidation with absorbance maximum at 234 nm

Dear Joan Carles Escolà-Gil

Thanks for ur protocol.

There are couple ways to oxidized LDL from the plasma, e.g. CuSO4, UV light exposure. First of all, you need isolate LDL, and then oxidize it. Here is the summary of my protocol for you ref, our lab have used this for more than 20 years and it works great. You can also find the papers in my page.(2012 Fu et al, HOG-LDL induced ER stress in HRCP , Diabetologia 55 3128)

10 μmol/l CuCl2 (24 h, 37°C), followed by repeated dialysis (4°C, 24 h).Protein content in LDL preparations was determined by BCA protein assay (Pierce, Rockford, IL, USA). LDL preparations were further characterised by measuring fluorescence at 360 nm (excitation) and 430 nm (emission) (Fluorimeter IV; Gilford, Oberlin, OH, USA), performing agarose gel electrophoresis (Paragon Lipo Gel; Beckman, Fullerton, CA, USA) and measuring absorbance (234 nm; DU 650 Spectrophotometer; Beckman). Preparations

were stored in the dark under nitrogen at 4°C in the presence of 270 μmol/l EDTA and were used within 6 weeks.

Dear Dongxu Fu ,

Thank you so much for valuable information and papers detail.