Hiii. I have phage 5' for E.coli ATCC 13706. I tried to increase phage concentration by method: 50 ml phage 108 PFU/ml + 10 ml of E.coli + 150 ml of PYCa medium, after incubating at 37 oC for 12 h, little clear medium can be seen, and the concentration of phage is around 108 PFU/ml.

Do you know any method to increase phage concentration to 1011 or 1012 PFU/ml? please let me know. Thank you very much.

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