I am currently trying to prepare metagenomic libraries using the low copy number cosmid pLAFR3 . However, the concentration of the plasmid when isolating it is extremely low, so I need 2 l of culture to get enough plasmid for one reaction. The cosmid has no tra-genes for induction. Is anyone aware of a possibility to increase the yield or has an optimized protocol for the isolation? Any suggestion/help is appreciated.

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