So I currently have 2 RNA extraction protocols, the Tempus RNA extraction using tempus tubes and an in-house Trizol/chloroform based bead-beating protocol for isolating M. tuberculosis RNA.

I was wondering if there would be a feasible way to incorporate these 2 protocols. e.g. using whole blood stabilised in tempus tubes and remove the supernatant containing the potent stabilising agent post centrifuge and add trizol/chloroform to the pellet and proceed to bead-beating?

Thank you so much for reading my question!

Ruobing