Amended on 29 September 2018

Genetic database seems to be depended upon non quantitative assay. Genetical or DNA analysing method such as PCR is not quantitative and unreliable.

Therefore, reliable data are obtainable by using new proteomics PDMD method (please see files; Tremella 2D-EP and HepG2 Fucoidan).

Famous Late Prof. Dr. Kunio Yamauchi (Department of Agricultural Chemistry, The University of Tokyo, Yayoi-cho, Bunko-ku, Tokyo, Japan) has found that α- S2 Casein and β- Lactoglobulin genes are absent in humans.

Italian Pediatricians Bertino E et al. have shown it in "Acta Biomed Ateneo Parmense. 1997;68 Suppl 1:15-9. Absence in human milk of bovine beta-lactoglobulin ingested by the mother. Unreliability of ELISA measurements. Bertino E, Coscia A, Costa S, Farinasso D, Prandi G, Fabris C, Cavaletto M, Giuffrida MG, Conti A., Cattedra di Neonatologia, Università di Torino, Italy". This paper also indicated the unreliable or non-quantitative nature of ELISA method.

I have quantitatively proven the presence of α- S2 Casein at 50.3 μg/mg of milk protein and β- Lactoglobulin at 27.5 μg/mg of milk protein only in the bovine milk by PDMD method (my unpublished observation).

Further, I have recently found that milk biotinidase is present in human breast milk at 5.5 μg/mg of milk protein, but absent in bovine milk (please see file; The Fascio effect).

Furthermore, major component proteins of human breast milk and bovine milk are shown as a reference (our unpublished result).

Species differences between humans and bovine seem to be very big.

Bovine milk

1st V-type proton ATPase 116 kDa subunit a isoform 1/Vacuolar proton pump subunit 1/Vacuolar adenosine triphosphatase subunit Ac116 at 126.4 μg/mg of milk protein

2nd Solute carrier family 43 member 3/SLC43A3 at 124.9

3rd α-Lactalbumin/Lactose synthase B protein at 110.0

4th Negative regulator of reactive oxygen species/Leucine-rich repeat-containing protein 33 at 104.3

5th α s1 Casein at 101.1

6th Aldehyde oxidase/Azaheterocycle hydroxylase 1 at 81.9

7th β- Casein at 78.8

8th κ- Casein at 74.3

9th Sialidase/Neuraminidase (Salmonella typhimurium) at 58.2

10th α- S2 Casein at 50.3

11th β- Lactoglobulin at 27.5

12th Fibrillin-1/MP340 at 15.3

Human breast milk

1st α-Lactalbumin/Lactose synthase B protein/Lysozyme-like protein 7 at 156.0

2nd β-Casein at 127.5

3rd Lactoferrin at 83.0

4th RING finger protein 86/Tripartite motif-containing protein 2/UBE2D1-dependent E3 ubiquitin-protein ligase at 55.0

4th Vacuolar protein sorting-associated protein 13B/Cohen syndrome protein 1 at 55.0

6th Major capsid protein/MCP (Saimiriine herpesvirus 2/SaHV-2) at 51.0

7th Protein Smp (Escherichia coli) at 40.3

8th Tetraspanin-14/Transmembrane 4 superfamily member 14 at 33.8

9th Lysozyme at 30.9

10th α s1 Casein at 30.0

11th MARCKS-related protein/Macrophage myristoylated alanine-rich C kinase substrate at 25.8

12th Human breast milk lipoamidase at 23.0

By the way, reliable PDMD method can foresee the origin of human kidney cancer.

Fetal hepatocyte Hc has kidney-type urinary-biotinidase (u-BIN) at 1.9 μg/mg of cell protein and has Genome polyprotein (Dengue virus/DENV; Flavivirus) at 6.9 μg/mg of cell protein.

Hepatoma HepG2 has no u-BIN, but has kidney-related proteins such as Uromodulin/THP/Tamm-Horsfall urinary glycoprotein at 2.0, and Uromodulin-like 1/Olfactorin at 0.32 μg/mg of cell protein, respectively. This cancer cell has Protein UL50 (Human cytomegallovirus/HHV) at 2.7 μg/mg of cell protein.

Healed hepatocyte HepG2 (cultured with fucoidan) has Protein KIBRA/Kidney and brain protein/HBeAg-binding protein 3/WW domain-containing protein 1 at 0.49, and u-BIN at 4.8 μg/mg of cell protein, respectively. This healed cell has Ribonucleotide reductase (HCMV/Human cytomegalovirus/Human herpesvirus 5/HHV-5) at 1.9, and Protein L (Mumps virus/MuV) at 2.2 μg/mg of cell protein, respectively.

Liver tissue (with pseudo liver cancer) has Collagen alpha 2 (IV) chain at 16.0, Cystic fibrosis transmembrane conductance regulator/CFTR/ATP-binding cassette sub-family C member 7 at 32.7, Sodium-glucose cotransporter 1/LC5A1 at 11.2, u-BIN at 16.0, Vasopressin V1a receptor/Antiuretic hormone receptor 1a at 1.7 μg/mg of tissue protein, respectively. Total kidney-related proteins become to be 77.6 μg/mg of tissue protein (7.8% among liver tissue proteins).

This normal liver tissue has Hypothetical protein HHLF3 (Human cytomegalovirus/HHV-5) at 4.9, Nucleocapsid protein (Mumps virus/MuV) at 1.8, RNA-directed RNA polymerase L (Measles virus/MeV) at 52.7, Genome polyprotein (Japanese encephalitis virus/JEV; Flavivirus) at 28.0, and Genome polyprotein (Dengue virus/DENV; Flavivirus) at 5.2 μg/mg of tissue protein, respectively. Total kidney-related proteins become to be 92.6 μg/mg of tissue protein (9.3% among liver tissue proteins).

HCC tissue (withPBC) has u-BIN at 8.3 μg/mg of tissue protein, and has 64 KD Lower matrix phosphoprotein (Human cytomegalovirus/HHV-5) at 7.8. and Hemagglutinin-neuraminidase (Newcastle disease virus/NDV) at 7.5 μg/mg of tissue protein, respectively.

LC tissue (named as No.6) has u-BIN at 15.2, Wilms' tumor protein/WT33 at 1.8, Collagen alpha 5(IV) chain at 12.5, and cAMP-dependent protein kinase type I-beta regulatory chain at 2.4 μg/mg of tissue protein, respectively. Total kidney-related proteins become to be 31.9 μg/mg of tissue protein (3.2% among liver tissue proteins).

This tissue has RNA-directed RNA polymerase L (Simian virus 41/SV41) at 20.1, L protein (Marburg virus/MARV) at 31.0, Hypothetical protein UL17 (Human cytomegalovirus/HHV-5) at 9.1, Genome polyprotein (Louping ill virus/LIV; Flavivirus) at 6.4, and Genome polyprotein (Dengue virus/DENV; Flavivirus) at 0.31 μg/mg of tissue protein, respectively. Total up-regulating proteins become to be 66.91 μg/mg of tissue protein (6.7% among liver tissue proteins).

This tissue has repressing virus of 3-Beta hydroxy-5-ene steroid dehydrogenase (Vaccinia virus/VACV) at 7.7, and Gene 20 protein (Herpes simplex virus/HHV-1) at 27.2 μg/mg of tissue protein, respectively. Total down-regulating proteins become to be 34.9 μg/mg of tissue protein (3.5% among liver tissue proteins).

Therefore, up-regulating protens minus down-regulating proteinsbecome to be 32.0 μg/mg of tissue protein (3.2% among liver proteins).

HCC tissue (No.6) has u-BIN at 9.5, Multidrug resistance protein 1/P-glycoprotein 1 at 1.0 μg/mg of tissue protein, respectively. 　　　　　　　　　　　　　　　

This tissue has up-regulating proteins of Structural polyprotein (O'nyong Nyong virus; ONNV) at 18.8, Non-structural polyprotein (Venezuelan equine encephalitis virus; VEEV) at 0.77, RNA-directed RNA polymerase L (Measles virus; MeV) at 18.433, Hypothetical protein UL87 (Human cytomegalovirus/HHV-5) at 8.5, and Genome polyprotein (Dengue virus/DENV; Flavivirus) at 19.8 μg/mg of tissue protein, respectively. Total up-regulating proteins become to be 66.303 μg/mg of tissue protein (6.6% among liver tissue proteins).

This tissue has down-regulating virus of Protein F1 (Vaccinia virus/VACV) at 24.5, and HHVs' protein (except HHV-5) at 25.5 μg/mg of tissue protein, respectively. Total down-regulating proteins become to be 50.0 μg/mg of tissue protein (5.0% among liver tissue proteins). Therefore, up-regulating protens minus down-regulating proteins become to be 16.3 μg/mg of tissue protein (1.6% among liver proteins).　

Since HCC is occurring by co-infection of HCV and HIV-1, human kidney cancer seems to be occurred by the co-infection of Flavivirus of

Dengue virus/DENV, Louping ill virus/LIV, Marburg virus/MARV, or Japanese encephalitis virus/JEV, and HIV-1.