Smering in Pcr Amplification ?

I am facing a problem regarding smearing in PCR amplification since two months. But, before six months the results was there using the same primers at 67.2-degree celsius annealing temperature, 5%...

23 November 2020 4,394 5 View

Dear all, I am interested in a project relating to agricultural waste management. Is there anyone who is interested to collaborate?

In this project on agricultural waste management, we will discuss the problem of agricultural waste, how it can be managed, and its legal framework. Area of study: Distt Patiala and Ludhina,...

13 July 2020 8,112 4 View

Whom should I approach for analysis and assembly of NGS reads of nanobodies in the camel phage display library?

We constructed Nb-phage display library derived from an LPS-immunized Indian desert camel and have recently subjected it to NGS using Illumina MiSeqTM platform. The raw data of NGS reads was...

24 May 2020 8,054 3 View

I am getting error saying could not open conf.txt for reading in AutoDock Vina.?

When I tried to docking windows cmd shows Error: could not open "conf.txt" for reading. I have tried to change the path for cmd e.c.t, but it was not helping. And I tried to change from program...

10 May 2020 8,821 4 View

Determining encapsulation efficiency of Liposomes using Triton X-100??

I am trying to determine the encapsulation efficiency of liposomes using Triton-X 100 in order to disrupt the membrane followed by extraction of lipophilic drug using hexane..Whenever i put hexane...

30 January 2020 3,404 3 View

How to change the intracellular pH in human cell line?

Hi All, I want to study the effect of change in intracellular pH. Can you suggest, how can I change the intracellular pH in human cell line. Is there any specific cell line suitable for such...

17 July 2019 1,054 1 View

How many iterations are generally required to get convergence for PEMFC simulations in ANSYS FLUENT ?

14 February 2019 849 4 View

Where can i find PCG(Phonocardiogram) with ECG(Electrocardiogram) database?

I found some ECG along with PCG from Physionet 2016 challenge dataset with name "a". But, description of ECG is unknown hence i am unable to apply particular algorithm to detect important...

24 December 2018 1,017 5 View

Where can i find waveletScattering function used in matlab?

I am currently using Matlab 2018a while i search help for waveletscattering it shows "waveletScattering not found" . Or is it compitable only in new version i.e Matlab 2018b.

23 December 2018 3,665 4 View

What is the difference between Bulk Metallic Glass and High Entropy Alloy?

What is the Difference between Bulk Metallic Glass (BMG) and High Entropy Alloy (HEA) with respect to a specific parameters (no. of elements, Purity of constituting elements etc.). Also,...

11 December 2018 8,793 7 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

How can I generate random samples from Bingham distribution in R? How can I construct those symmetric matrices?

in the function rbingham(n,A) in R software how can I construct that A (symmetric) matrix?

24 February 2021 3,528 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Multinomial probabilities and confidence intervals - obtaining the CI of the reference category?

Hi, Some background to my question: I have fitted 1st order multinomial Markov models with covariates. Since all probabiilties (for each row of the transition matrix) sum to 1, I estimate only...

22 February 2021 2,541 4 View