I aim to detect and identify nitrated and nitrosylated proteins in a particular cell type. These modifications, especially nitrosylation, is highly reversible. I have following 3 questions:
1. Is there a way to treat cells with "something" so that the proteins dont lose nitration/nitrosylation during time point collection, lysate preparation, and IP?
2. are there any good antibodies to IP nitrated Tyrosine, Cysteine, Tryptophan ?
3. Except Biotin switch, any other good method to detect nitrosylation?
Thanks
Hi Sanjay!
You can use S-nitrosylation kit from Pierce instead. This kit works really well.
Hi Rajeev, Thank you for this info. I basically have to avoid de-nitrosylation and then also identify the proteins which were nitrosylated/nitrated. I guess this kit will tell me the global status of nitrosylation, not about the individual proteins.
Hi Sanjay,
This publication may be of help to you:
Article Protein S-nitrosylation: A physiological signal for neuronal...
All the best,
Hediye.
Thank you for the paper. I will read it and let you know. Best wishes.
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioecology
- Systems Ecology
- Landscape Ecology
- Connectivity