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Questions related from Sanjay Premi
Hi all, I have one cell type treated with 3 drugs, 4 sample in total: Untreated, Drug 1, Drug 2, and (Drug 1+2). There are two time points post treatment. The aim of the experiment is to prove...
13 April 2021 779 0 View
I aim to detect and identify nitrated and nitrosylated proteins in a particular cell type. These modifications, especially nitrosylation, is highly reversible. I have following 3 questions: 1....
10 March 2018 4,122 4 View
My experiment is to make dsDNA from a pool of variable size fragments (~200 bp to 25 kb). I am using degenerate decamers for second strand synthesis with Phi 29 polymerase. Next step is to purify...
10 January 2016 3,055 4 View
I want to make double stranded DNA out of single strands using random decamers or other lengths of random oligos. The conditions is that the random oligos should space apart about 1 kb to 1.5 kb...
18 May 2015 4,905 6 View
I expect a 70 bp amplicon which shows up only with 2% (or higher) DMSO in the PCR. But with DMSO, the size of this amplicon goes from 70 bp to ~110b bp on 3% agarose gel. Same happens when a...
03 October 2014 456 4 View
I am trying to make blunt ends out of nicked plasmid DNA using random hexamers and Klenow polymerase (large fragment) from NEB which has 3'-5' exo but no 5'-3' exonuclease activity. My goal later...
03 September 2014 3,856 4 View
I am trying to cut ~ 5ug of pUC19 plasmid, exposed to very high UVC dose (~800 J/m2) using T4 endonuclease V from NEB. Everything is being done according to NEB Protocol. I tried increasing the...
11 June 2014 3,973 5 View
I am getting very high 260/280 ratios after a phenol chloroform extraction of small sized DNA fragments. Extra care was taken not to leave any phenol or chloroform in the DNA solution before...
12 May 2014 441 6 View
