Dear all,
I have paired-end raw reads of Nicotiana tabacum (SRA: SRR9191672) and their reference genome which is available on NCBI. so after fetching the data, i decided to map the reads with reference genome with the help of bowtie2 and after successfully running bowtie2, I got multiple index files like.. genome.btindex.1.bt2, genome.btindex.2.bt2, genome.btindex.3.bt2, genome.btindex.4.bt2, genome.btindex.rev.1.bt2, and genome.btindex.rev.2.bt2. so please let me know which one is my actual genome index file for my further downstream analysis.
thank you.
Dear Aqib Sarfraz
These links might be helpful, have a look:
1. https://www.biostars.org/p/372511/
2. http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml
Kind Regards
Qamar Ul Islam
Dear Qamar Ul Islam,
thank you for the links you provided, and the time you have spent on the same...
the problem is now solved, thank you once again
- Badges
- Science topic
- Similar topics
- Filing