How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

SAR X-Band constellation from Capella Space?

Dear all, I'd like to open here a sort of forum for understanding how the geodesists community is moving in view of the X-band SAR satellite constellation. The new constellation will offer new...

24 January 2021 6,248 0 View

Colab - how to extract NDVI time series from Sentinel-2?

Hi all, I have a collection of Sentinel-2 images and several points within it. In Google Colab, I would like to extract NDVI time series over these points through the whole collection and display...

12 January 2021 4,044 3 View

The use of Orange, a high level tookit for Machine Learning, data mining and data science for scientific research or just teaching?

Related to data mining/data science, I recently discovered Orange (https://orange.biolab.si/) a high level very powerful free toolkit. Using it is very simple and you can test different models in...

09 December 2020 8,832 4 View

How to defend against the reviewer's "not enough trials" objection (non-parametric observed power issues)?

Hi everyone, we need some help with a request from a reviewer. Here's the issue: In our study we investigated the impairment in the ability to recognize facial emotions when a facemask is present...

05 November 2020 1,238 18 View

Any difference between "hydroxy naphtol blue", and "hydroxy naphtol blue , disodium salt" for LAMP?

I am reproducing this work on LAMP (isothermal amplification) A new visually improved and sensitive loop mediated isotherm... , but I am a bit confused about the difference between "hydroxy...

31 August 2020 7,289 3 View

Estimate Enterprise Value using specific accounting (items/lines) variables in regressions?

Need help in finding the best papers related to the above question. Thank you.

30 August 2020 8,430 12 View

Effects of grammatical phrasing (2nd person singular vs. 1st person plural) on compliance to suggestions/prohibitions/prescriptions, etc?

Hi guys! I have a question on a framing effect-like issue. Well, everyone of us has the immediate feeling that there's a huge difference between saying - for instance - "you should respect the...

20 August 2020 7,859 6 View

How can particle fillers increase the elongation at break of polymers?

I am investigating some biocomposites formed by PBAT and tiny particle fillers derived from natural wastes. I have noticed that with a loading of 10%wt. of these fillers, the elongation at break...

28 July 2020 9,367 1 View

How to change features size without padding in CNN?

Hi, I'm using a 2d CNN. My input size is a square image of 20x20 pixels and my desired output has size 400x3. The most common thing I see is to introduce padding over the convolutional layers...

20 April 2020 2,947 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View