Hey everyone,

I'm trying to do whole-cell recording from CA1 dendrites (about 200 um away from soma or more).

I got a 60x objective recently to have a better visualization. But it seems like the dendrites start to become thin pretty much early. How can I patch them?

I have seen people saying to lower the pipette from above but it seems like the dendrites are thinner than my patch-pipette tip. how about coming from the side a better approach?

Also, I'm not great at recognizing which dendrite is healthy. Does anyone have some examples of how healthy dendrites look like?

Thanks.