How to get rid of MAP clumps without sonication?
You should add some mild detergent such as Tween 20 to your culture media in tiny proportions to get rid of this.
Thank you very much for your response.
Herein, you may find another solutions for dispersing MAP clumps:
1) Vortexing the MAP suspension with glass beads (3 mm) for 40 seconds
2) Homogenizing the MAP suspension by ribolyzer using glass beads for 4 cycles of 45 s at 4 m/s.
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...
22 May 2024 1,334 0 View
In my undergraduate research project, I require an integrative plasmid containing GFP with an antibiotic resistance that can't be kanamycin. In our lab, all of our integrative-GFP-containing...
14 May 2024 9,861 3 View
I am trying to express a FAD containing enzyme of mycobacteria in E.coli. I am able to purify the protein which is slightly yellow in color but it seems that my FAD is all unbound to the protein....
16 April 2024 6,912 1 View
I am performing a bacterial transformation on Mycobacterium abscessus spp. abscessus using pMSP12::mCherry. I need to know if this plasmid is integrative or replicative
01 April 2024 8,366 3 View
I am doing a resazurin assay to determine the MIC of plant extracts against Mycobacterium tuberculosis. The problem is the MIC is coming at a very high value. I read in one paper that even if...
01 January 2024 7,916 2 View
I would like to know the species-specific probe targets of GenoType NTM-DR for mycobacteria. I need this information to compare with the sequence-based...
29 November 2023 7,215 0 View
Bacteriology
24 October 2023 7,903 10 View
I tried to do same with membrane fraction. It expressed in membrane but somehow not working in periplasm
14 September 2023 2,109 0 View
Merhaba değerli hocalarım ben Melisa, Mersin Üniversitesi Moleküler Mikrobiyoloji bölümü yüksek lisans yapmaktadır. Tezimde Mycobacterium türlerini çalışıyor. Bütçenin kısıtlı olmasından dolayı...
20 August 2023 2,188 0 View