Different primers for same gene but different results in expression?

Hey everyone, I am currently doing my doctoral thesis at the department of pathology. I designed two different primers for my gene of interest having two different amplification sizes (130bp and...

17 February 2021 6,684 3 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

How to address SRMR (0.2 to 0.8) to make model fit?

Dear researchers, I want to update my knowledge regarding data analysis. I will be honored if you can help me to reduce in correcting SRMR in SEM_PLS. All the measures (AVE, CR, VIF, CA, HTMT) are...

09 February 2021 1,702 1 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Are ther ways to quantify / measure the degree of loneliness?

Are there ways to quantify / measure / monitor the degree of loneliness of team members in a Covid-19 lockdown teleworking situation? Team dynamics over a longer period of teleworking and...

16 November 2020 4,212 9 View

Does anybody have experiences with FFF separation of PHB particles?

I am trying to separate suspension of PHB particles, stabilized with PVA. The DLS results show two populations with peak maxima about 150 nm and 380 nm. The Zeta potential was -3 mV (pH 7) across...

21 October 2020 6,551 1 View

What is the impact of the covid-19 pandemic on global warming?

Because of extensive lock up during the pandemic in many countries of the world, notably Europe, USA, Brazil, China and India, there was reduced use of hydrocarbons, especially due to much reduced...

15 October 2020 8,539 23 View

Is there any way to find RUL of bearing with incomplete dataset?

I am interested in finding RUL of bearing, which is currently in operation. I could find papers which uses run to failure datasets, which is not of my interest. Can anyone suggest methods or...

11 October 2020 7,962 7 View

Does anyone know of ontologies of the knowledge covered in US middle school and high school curricula?

For one of our projects we are looking for ontologies of middle and high school topics, such as math, science, history, and others. Is anyone aware of existing ontologies that are available for use?

10 October 2020 2,040 3 View

Multiple-Group Invariance with Categorical Outcomes AND correlated residuals?

Dear all, I have a question relating to the following paper: Dubravka Svetina, Leslie Rutkowski & David Rutkowski (2020) Multiple-Group Invariance with Categorical Outcomes Using Updated...

08 October 2020 3,142 3 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

Is there any relevant literature detailing extraction methods of simple sugars from plant material for LC-MS analysis?

For my research, I would like to extract glucose, sucrose, and fructose from the crowns of strawberry plants and quantify these sugars using LC-MS. However, I have not had much luck finding a...

11 February 2020 398 3 View

Methanolysis and HPLC measurement?

I would like to perform methanolysis of proteins to get the monosaccharide building blocks. After methanolysis the monosaccharides are present as methyl glucosides - how can I analyze them by...

13 February 2019 9,591 1 View

Methanolysis and HPLC measurement?

I would like to perform methanolysis of proteins to get the monosaccharide building blocks. After methanolysis the monosaccharides are present as methyl glucosides - how can I analyze them by...

01 February 2019 758 1 View

How to remove acetic acid from solution containing glucose, xylose and acetic acid?

Hello, I would like to know if there is a method to remove acetic acid (around 3g/L) from an aqueous solution containing Glucose, Xylose and acetic acid. I just want glucose and xylose in...

05 June 2018 8,012 4 View

A good reference about thermodynamics of glycosidic bonds?

08 September 2017 3,726 3 View

Is there any possibility of determining the type of monosaccharides from proton, 13 C and DEPT spectrums?

or GCMS, TLC are the only way to determine the monosaccharide presence

03 April 2017 5,956 5 View

NP-HPLC for monosaccharides composition analysis with fluorescence detector?

07 August 2016 1,373 1 View

Can yeast mannan oligosaccharide enhance hemagglutination activity of a lectin?

We are trying to express a lectin in E.coli, which is specific to high mannose glycans. Using akta system and size exclusion column (Sephacryl s-200), we isolated protein though at a pretty low...

04 May 2015 4,599 1 View

Protocol for synthesis of selectively protected monosaccharides?

Dear all, for a synthesis, we need 4'-OH-Monosaccharides (all other hydroxyl-groups should be protected, acetylated or benzylated or else...) - I am desperately looking for a working protocol to...

11 December 2014 4,617 8 View