Dear all,

I am trying to create a lentivirus construct to insert in colon cancer cell that express a GFP with conditional fluorescence.

I have found several methods that use GFP beta-sheets 10 and 11 separated and linked with small peptides (connected with DEVD caspase-3 specific cleavage sequence)

I am trying to create a stable cell line with this lentivirus to study xenografts in nude mice. Ideally GFP signal should be revealed after chemotherapeutic treatments.

In short: How to create stably infected tumour cell lines with GFP reporter activated by caspase-3 cleavage that can be observed in Xenograft?