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Questions related from Harry Nie
Hi all, I set up the crystallization of protein-DNA complex for initial screen and found one crystal cluster appeared in a condition (0.1M HEPES pH7.0, 0.2CaCl2·2H2O, 45% MPD). Afterwards I added...
05 May 2023 5,082 1 View
I set up the crystallization of protein-DNA complex for initial screen and found the condition to optimize (0.1M HEPES pH6.5, 0.2MgCl2, 25% PEG3350). After rounds of optimization, the quality of...
14 April 2023 5,167 0 View
Hi all, Recently I am working on protein purification. 1. affinity column(GST) 2. Add PSP enzyme to remove GST-tag(O/N) 3. GST-tag off 4. gel filtration However, the protein tends to form large...
08 November 2022 9,403 2 View
Dear all, I meet a big trouble in ChIP assay. I prepared the cell lysis buffer/shearing buffer containing 1%SDS which helps sonication well. However, 1%SDS damages the epitope of target protein...
25 June 2021 3,733 1 View
