Hi, I'm trying to functionalize DFNS (dendritic fibrous nano silica particles) I got from a project partner with APTES, GPTMS and OTS to gain amino groups, carboxy groups (after addition of succinc anhydride) and octadecyl chains. So I made an FTIR spectrum before the treatment with the silanes and after that. I added the spectra as PDF files. My method:
I suspended 10 mg particles in 1 mL anhydrous toluole, sonicated it for 15 minutes, vortexed it for 5 minutes and added APTES/GPTMS/OTS with a final concentration of 1 %. After that I incubated it at 25°C, 1000 RPM in ThermoMixer for 24 h. I treated the GPTMS functionalized particles with succinic anhydride 2 h, 60°C, 1000 RPM.I washed all particles 3 x with Toluol and dried the centrifuged particles at 70°C and then I made the FTIR.
I'm new to this silane chemistry and my two main questions are:
1. Are there silanole groups on the surface of the particles because I'm uncertain how to read the FTIR spectrum correctly
2. Did the functionalization work because I think there is not really a peak for amino or carboxy groups.
I'm sorry if I made a basic mistake but I'm from biotechnology and this whole stuff about silanes is very new for me.
I hope somebody can help me.
To check for silanole groups in your FTIR spectrum, look for a broad peak around 3200-3600 cm⁻¹. For successful functionalization, verify the presence of amino groups (3300-3400 cm⁻¹) and carboxy groups (1700-1720 cm⁻¹); their absence may indicate ineffective functionalization.
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