Sergio López-Herrador

2 Questions 1 Answers 0 Followers

Questions related from Sergio López-Herrador

Why Is My mtDNA Ct Value Higher Than Nuclear DNA in qPCR?

Hello everyone, I'm currently working on calculating the relative levels of mtDNA using qPCR by comparing the Ct values of a mitochondrial gene (nd1) and a nuclear gene. According to my...

17 July 2024 5,507 0 View

How to Freeze Embryos for Spatial Transcriptomics?

Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...

11 July 2024 5,984 0 View