Is there any reason why I can not order an oligo and use it directly as an insert for SLiCE cloning?

I was about to design the primers for SLiCE, when I realized the insert is so small I could order it as one oligo and it would not even be that expansive. As its quite some time ago, that I used...

18 March 2020 2,996 3 View

Ethically, what are your views on publicly revealing reviewer status after publication of the journal article?

I generally was taught that reviewers should keep their reviews confidential forever. I was invited to review an article that was recently published. It was then picked up by a major news source...

19 December 2019 9,463 5 View

Required Sample Size: Full Profile Conjoint Experiment?

Hi all, I am trying to compute a minimum required sample for conjoint experiment where every participant sees each attribute combination represented in a profile. I am planning for four to five...

03 December 2019 8,904 2 View

Looking for breastfeeding/infant policy statement or practice guidance from dietetic associations?

I have a policy statement from the British Dietetic Association, and both a policy statement and practice guidance from the Academy of Nutrition and Dietetics (USA). I can't find any other...

22 October 2019 892 10 View

Why are poetry and literary journals that publish it not in your data base?

I publish poetry in peer reviewed journals. These do not seem to be in your "data base."

05 January 2019 5,175 3 View

Are there any studies that explore the relationship between cuticle thickness and body size in species of decapod crustaceans?

This relationship is very obvious to me (think of lobsters), but hard to actually measure. I could not find any study which addresses this issue!

11 September 2017 1,995 14 View

Best way to clean CTFE equipment in order to avoid DNA contamination?

Dear colleagues, I received different pieces of lab equipment (mainly pestils and alike) out of CTFE and would like to reuse the same pestils without autoclaving them every time. I do not have any...

29 August 2017 4,356 2 View

What are the systems responsible for regulation and expression of behavior in birds?

06 January 2017 320 5 View

Optimization problem for PGLS in gls in R?

16 March 2016 3,942 2 View

How do I stop RIPA-insoluble, Urea-soluble protein fraction from precipitating in Western blot wells?

18 November 2015 2,156 7 View

How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

How to identify when a gaussian program is about terminate normally?

Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...

05 February 2021 8,721 3 View

What is the reason for using Glass Impingers?

Impinger bottles are used at some applications, where the gas is cooled and the condensate is dissolved in a solvent contained in the impingers. I have always seen impingers made of glass. Why not...

03 February 2021 6,959 2 View

How to design an Ultrafiltration membrane for a CSTR?

Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...

27 January 2021 7,924 1 View

How can I remove bubble in WPU film?

I am trying to prepare water-based polyurethane films. When dried air bubbles were formed and destroys the film homogeneity, then I can't use this for mechanical test because of defects. Is there...

26 January 2021 701 1 View

How can I get rid of the bubbles in the SBS membrane?

I used methylcyclohexane/ ethylcyclohexane / n-butyl acetate mixed solvent to dissolve thermoplastic SBS & terpene resin. The mixture is then poured onto a glass substrate. After solvent...

24 January 2021 8,306 3 View

Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

Where can I find these two papers?

14 January 2021 1,572 3 View

How to model bubble coalescence and/or departure from a surface in ANSYS Fluent?

Dear all, I would like to simulate the pool boiling (subcooled). Specifically, I'm interested in departure of bubbles from finned surfaces. I want to do it in ANSYS-Fluent. Is it possible? if so,...

14 January 2021 6,304 5 View

How can I use Non-dominated Strong Genetic algorithm(NSGA-II) for optimization?

I have a range of gas stream temperature, and I wanted to get the optimum temperature in the gas compressors.

14 January 2021 3,180 3 View