i have sequenced the mycobacterium pncA gene in drug resistance strains and got a number of mutation after sequencing.. now i do not know how to find which mutations are novel? there is any tool or software that can find that the mutation is novel?
Muhammad Umair
Hi,
You can search for literature using https://www.ncbi.nlm.nih.gov/pubmed
or look into the public data base using http://exac.broadinstitute.org/
or search using http://www.hgmd.cf.ac.uk/ac/index.php to find if the mutation is not already reported.
Best wishes.
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