I have separated FAME (fatty acid methyl ester) biodiesel on TLC using hexane and ethyl acetate ( 9:1). can i now use the same solvent hexane and ethyl acetate mix for column chromatography. please suggest and appropriate answer.
TLC is routinely used in finding the appropriate conditions for column chromatography separations, provided that the same sorbent is used for both. Hexane/ethyl acetate mixture is very common for silica gel columns, and the only needed adjustment would be the hexane-ethyl acetate ratio to get an optimum Rf for separation.
Usually the Rf between 0.3-0.5 for the desired spot works best, but it depends on the spots separation, elution order, relative column size and, occasionally, some other factors.
Dear Sir. Concerning your issue about the suitable solvent for column chromatography for separation of fatty acid methyl ester biodiesel . As indicated by Lin et al. indicated that silica gel and hexane-chloroform-diethyl ether-acetic acid (80 : 10 : 10 : 1, v/v/v/v) were good in analysis of docosahexaenoic acid (22 : 6 DHA) in the spermatozoa of monkeys. Individual phospholipids from this sample were separated by another system such as chloroform-methanol-petroleum ether-acetic acid-boric acid (40 : 20 : 30 : 10 : 1.8, v/v/v/v/v). For more detais, I think the following below links may help you in your analysis:
https://www.hindawi.com/journals/jchem/2015/120830/
Thanks
Ajaj
if you got a good separation on TLC, most probably you will get a good separation on column chromatography. For best results, use silica of small particle size (i.e. 40-60 um) and previously optimize your solvent mixture in TLC adjusting hexane/ethyl acetate proportion until you obtain Rf values of mixture components that differ appreciably
thank u Vladimir Khlebnikov, Isam Eldin Hussein Elgailani, Sebastián Finger
"Analysis of biodiesel by high performance liquid chromatography using refractive index detector"
by Mahin Basha Syed
downloadable:
https://www.sciencedirect.com/science/journal/22150161
Regards
Detlef.
Reference [3] of this article might be of big interest for you, as it describes the procedure in great detail.
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