I mean is there any database which collects these biophysical information ?
Well, database may not be spcecific for your particular enzyme molecule. while searching for any property of the molecule be very careful about the mentioned source of the enzyme. These are inherient properties of the a molecule which depends upon a no. of factors like the total charge, amino acid composition,amount and type of sugar residues present, disulphide bonds, over all conformation of protein etc. etc.
So its always a best bet to check for pH and temp experimentally. What you can do is to standardize or find out any functional assay for your enzyme and then check for the retention of activity at different temp and pH , changing only one parameter at a time. For example, check for enzyme activity at different pH, say, 5.0 . Again, check for pH 6 while maintaining all other parameters same like before except pH which is 6.0 now, instead of 5.0. Same way check for all the desired pH range and temperature. Plot the activity vs pH/temp curve and the highest point is your optimum, You may get a broard optimum from your graph, which reflects the overall working range of your enzyme .
Hope this may help
Make an experiment with pH and Temperature the different variables. Test each one individually and see what factors increase reactivity of the enzyme. Once you figure out the best scenario for each variable. Attempt both variables simultaneously to be sure that the variables do not counteract with each other.
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