What is the best ratio for practical skills and theory for full time students?

When teaching vocational subjects we have to balance between theory and practical skills for students to progress to next level.

19 May 2020 4,367 3 View

Minimum levels of COVID detection?

Hello good people, This is not a normal question for me, but a friend in Public Health wanted to know, so I thought I would ask. The test for COVID is a PCR, if I am not mistaken. The question...

16 March 2020 3,322 2 View

How to clone a gene with long sequence?

Dear All, I'm now trying to amplify a gene with 3690bp from the genomic DNA. I've design 3 sets of primers. First set is flanking the whole gene. Second set is flanking from 1 to 2613bp. Final...

07 February 2019 1,167 1 View

How do I remove something that publisher complained about?

I cannot share copyright book. Mary Ellen Gioldberg

19 January 2019 7,782 3 View

Has anyone examined the ferret pup retinal vasculature?

I cannot find any literature. Is it similar to the mouse? Rat? Human? Has anyone tried it as a model of retinopathy?

14 January 2019 1,457 2 View

Looking for ways to include water quality microbiological parameters into a statistical analysis using censored data analysis methods?

Interested in finding out other peoples experiences in dealing with microbiological parameters (such as Faecal coliforms, total coliforms, etc) are are below the method detection limit of the...

12 January 2018 7,546 2 View

Anybody have resources (article) for predicting via logistic regression modelling for an environmental contaminant above a threshold concentration?

Environmental Logistic regression modelling Contaminant Machine learning

29 December 2017 1,741 4 View

Good protocol for DNA/RNA Immunoprecipitation (DRIP) detecting R-loops?

I'm trying to do a DNA/RNA immunoprecipitation (DRIP) using the S9.6 antibody and am getting no DNA pull down. I isolate DNA from cultured cells using the Qiagen DNeasy Blood & Tissue Kit and...

07 September 2017 148 3 View

What do you think of rapid curriculum change?

In the philippines, the 10-year basic education has changed to Kto12 curriculum. i need to know the issues behind rapid curriculum change and the things needed to prepare for such change.

16 February 2017 9,169 12 View

Does anyone have experience or advice on analyzing a population?

We have 8 years of data that includes info on all crimes reported to MPs and all crimes the commanders submitted for prosecution. This is not a sample - it is the entire population of crimes...

19 May 2016 347 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

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22 February 2021 9,932 11 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View