I want to identify the modification of lipid A by mass spectrum analysis. However, the extraction is tricky.
Several methods are suitable for the isolation of lipids, many of them based on the chloroform:Methanol mixtures. However, recently Matyash et al. developed a modification using MTBE what is a very good improvement to the original method. Furthermore, Sarafian et al. has also reported a very comparison of isolation methods suitable for UPLC-QTOF. These latter authors claim to have very good results using isopropanol.
In my experience, an isolation have to as simple as possible, fast and reliable. If you need more help, please contact me.
These above mentioned methods are very good if you are assaying total lipid analysis. However, if you are interested in fatty acid analysis you may use a direct derivatization method (Pimentel et al. 2016; Castro et al. 2014).
Good luck and good work.
http://www.jlr.org/content/49/5/1137.full
http://www.ncbi.nlm.nih.gov/pubmed/24820162
http://www.sciencedirect.com/science/article/pii/S2215016115000618
http://www.sciencedirect.com/science/article/pii/S0039914014004354
If you just looking for lipid A, you can also try just precipitate the proteins with ethanol, evaporate to dryness and extract the pellet with nonane. You can injecta few uL nonane directly into a high flow HILIC chromatography system.
You asked only about extraction, I assumed you have a LC-MS method to selectively detect lipid A.
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