Liang Huang

5 Questions 6 Answers 0 Followers

Questions related from Liang Huang

Is the the amino acid sequences similarity related to the affinity of proteins?

I was reading Errett C. Hobbsa 's paper, titled "Conserved small protein associates with the multidrug efflux pump AcrB and differentially affects antibiotic resistance" . In this paper the author...

05 May 2016 7,352 1 View

How to predicts if there is interaction between peptides in silicon?

Here we have two peptide sequences, one of them is not fully annotated, and the function is unknown. We want to know if there is interaction between peptides in silicon. Is there any...

05 May 2016 2,843 3 View

Is the Lac promoter inducible if the cloned gene has its own promoter ?

I knocked out my target gene, in order to confirm its function, I did the plasmid complement test. I cloned the gene with its full promoter into the MCS, however, it seems that the expression...

10 October 2015 5,627 7 View

Why does the partial digestion of the DNA to generate plasmid library not work?

I tried partial digestion for the genomic DNA (bacteria) by Sau3AI, I digested the DNA in a very short time (~ 30 mins), then the reaction generated a smear band in gel, I cutted the band (1-2kb,...

06 June 2015 9,200 3 View

How to extract the bacterial Lipid A for mass spectrum analysis?

I want to identify the modification of lipid A by mass spectrum analysis. However, the extraction is tricky.

01 January 1970 6,093 4 View