While extracting the active chemical in ethylacetate the yield was very, very low, less than 100mg/liter. In many publications I saw there is on an average 500mg or more yield per liter of culture filtrate (actinomycete) processed. Is there any step not mentioned in the publications? Can anyone suggest how to get more yield from the culture filtrate?
Ameriga Lazzarini
Did you use a pH buffer? Is your target molecule stable in the conditions employed? Do you know its structure and presence of acidic or basic functions?
Did you assay the total amount produced before starting the extraction?
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Ameriga Lazzarini
If your molecule lack polar groups it could remain adsorbed in the mycelium
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