What is the best way to evaporate diethyl ether from a mouse plasma extaraction? I am trying to extract indole from the mouse serum, using diethyl ether, and my sample size is very small (< 1 mL). Would it be possible to evaporate off the diethy ether by just putting it under a fume hood?
Our rotovap can fit 45 mL tubes at the smallest and it's not under a fume hood so I'd prefer to not have to use it. Would I need to evaporate under a flow of nitrogen?
Paul Milham
Most will evaporate. But if it needs to be absent you may then need to put it under vacuum
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