Dear RNA researchers
I would like to know the difference of transcription of Norad lncRNA between my control and knockdown cells. Would you recommend the appropriate method to evaluate transcriptional efficiencies in both cells? Initially, I thought that pre-mRNA qPCR (often used for mRNAs) is an appropriate one, which seems that it is not a good method for lncRNAs.
I appreciate if you would provide me with constructive suggestions.
Best regards,
Akiko
Dear Akiko Yanagiya,
I recommend reading the following articles to get the answer to your question.
Best Wishes,
Article Long noncoding RNAs in the p53 network
Article High long non-coding RNA NORAD expression predicts poor prog...
Hi Akiko Yanagiya,
I think you can combine both qRT-PCR with RNA-FISH, however the latter is more definitive that you successfully knocked your lncRNA down. You may consider Northern Blot as well that should showed a significant decrease in your KD samples.
I hope this help to answer your question
Good Luck
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