I am looking for a spectrophotometric assay to quantify total glycosides content in plant samples. Can you suggest any?
Can anybody share the experience with the antitryptic activity assay using botanical extracts? Which standard and in which concentrations should I use to obtain the standard curve? In my method,...
02 March 2018 10,021 1 View
I am estimating the total phenolic content (TPC) of 70% ethanol plant extract using the Folin-Ciocalteu method. My samples are rich in phenolics, so I need to dilute them to proper concentration...
08 September 2016 5,687 6 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
I want to extract glycosides from my mangrove leaf samples, but my laboratory lacks an incubator capable of reaching temperatures above 60°C. The paper I am following specifies a minimum...
13 July 2024 3,686 0 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View