we are planning to prepare an ointment, for which we have to study the rate of release of nitric oxide. so dear colleagues please suggest any methods
Dear you can proceed through membrane diffusion cell (KC Diffusion cell, Franz diffusion cell) etc.
Hi,
You can also use enhancer cells (modified USP2 dissolution model), see the following publication by Pankaj R. Rege and al: Development in release testing of topical dosage forms: Use of the Enhancer Cell(TM) with automated sampling. Journal of Pharmaceutical and Biomedical Analysis 17(8):1226-33, september 1998
For the quantification of nitric oxide you can use a method that determines nitric oxide concentrations based on the enzymatic conversion of nitrate to nitrite by nitrate reductase followed by colorimetric detection of nitrite (Griess Reaction) , you can find assay kit for determination of nitric oxide in biological samples (urine, serum, plasma..)
Best regards,
You can use a nitrate (perhaps they sell a nitric oxide electrode) selective electrode (ISE) to give you concentrations.
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