How to estimate the ATP and mitochondrial membrane potential from isolated mitochondria ?

Jitendra Narain Singh @Jitendra-Singh-14

23 August 2016 1 6K Report

I want to do ATP estimation and mitochondrial membrane potential of from isolated mitochondria from cells. We want to use dye with the plate reader. Can anyone suggest me the detailed procedure of the assay for estimating the ATP and membrane potential from isolated mitochondria?

Christian Q. Scheckhuber

I recommend to have a look at the publication in the attachment. It will help you with your question.

Regards, Christian

Article Chapter 20: Functional Assessment of Isolated Mitochondria In Vitro

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