We have attempted to reproduce a methodology to cultivate Aeguerita in soybean meal/peptone medium, but we consistently encounter contamination issues, apparently due to thermoresistant spores present in the soybean meal. Even after boiling the medium prior to sterilization and applying multiple autoclave cycles, the contamination persists.
Anyone thas found similar problems?
If the autoclave works properly, then no spores should be viable after sterilization. You can try use a different apparatus. If you can dissolve the medium you can filter sterilized it and then by autoclave. I suspect however, that the issue is either with the autoclave or the lab equipment (Petri dishes, pipettes) or air contamination. If the contamination if purely bacterial you can add some antibiotics like chloramphenicol.
We have sticker for autoclave temperature and its arrives to 121 ºC, this should be enought (with other media we do not have this problem). The problem is particular with this medium
How does this contamination look like? Maybe its just flocculation of the meal itself.
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