How to elutie V5-tagged target of interest?

01 July 2019 0 4K Report

Hi there,

I was planing to elute V5-tagged target of interest from anti-V5-tagged antibody beads. The heat denaturing approach would fit my needs. However, I was wondering if the antibody would fall off from the beads when heating at around 90 oC. Any suggestions and answer to that would be highly appreciated.

Thanks,

Chun

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