7 Questions 9 Answers 0 Followers
Questions related from Chun Guo
Hi there, I was planing to elute V5-tagged target of interest from anti-V5-tagged antibody beads. The heat denaturing approach would fit my needs. However, I was wondering if the antibody would...
01 July 2019 3,856 0 View
Hi there, I was wondering how to determine the substitution value(something like ~umole/g) for the resin in solid phase peptide synthesis(Fmoc chemistry). I have seen people mentioning about...
25 February 2019 4,263 6 View
Hi all, I was wondering how could I generate random strings with controlled bias. For instance, to generate various trimer sequences with distribution of A 50%, C 20%, G 20%, T 10%. Thanks, Chun
21 January 2019 2,313 4 View
Hi, Does anyone with experience with coupling two amine reactive handle using DST(disuccinimidyl tartrate)? I always get inconsistent and poor yield from that. Thanks
13 July 2017 8,810 2 View
Hi there, I plan to perform a SELEX on a protein target which has multiple binding site(at least two). Does anyone has experience with where can I find them? Thanks for your attention
29 April 2017 3,381 2 View
I am curious about the stability of a 2'-FANA single strand when comparing to a native DNA single strand. I know it is more stable than native RNA as the 2'-fuoro group distablized the...
24 March 2017 247 0 View
I decide to perform PCR on a non-natural nucleotide strand. The strand is composed of one modified region(40 non-natural nucleotides) and two primer sites(20 nts natural DNA for each). The...
24 March 2017 1,041 0 View
