Dear Dr. Vassilev, there are some methods to prevent biofilm formation on the surface of biomaterials. Coating, changing surface properties (e.g. surface roughness, charge, ionic properties), changing chemical properties and usage of antimicrobioal agenst to prevent growth of biofilm forming bacteria when they are in planktonic form.

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Dear Vassilev, besides above mentioned methods we can also use ozonation and low-energy surface acoustic waves.

Thanks

Dear Dr. Vassilev, generally, antimicrobial treatment alone is ineffective to remove biofilm. To remove biofilm, you should increase the penetration of the antimicrobials or use some physical method to loose the biofilm. If the medical devices are not big in size, ultrasonic + antimicrobials (sanitizer or disinfectants) are a good method to remove biofilm. Also, hot water treatment is very effective to kill bacteria protected by biofilm. And some antimicrobials in gas phase such as ozone and dioxide chlorine has good penetration to biofilm. To prevent biofilm formation, you should always use sanitizers or disinfectants to sanitize or disinfectant the devices that are suspiciously contaminated by pathogens at the initial stage. Hope this may help you

If possible, one may try to keep the device or the relevant parts immersed in an appropriate buffer so that the charge difference would remove some (if not all) the biofilm formed. The next method, which we normally follow in animal tissue culture is to give a protease treatment which might remove the biofilm.

Following Article helpful to you. It includes all of novel methods to remove of Biofilms in anywhere. Please read it. You will able to remove biofilm from your device.

Thank you.