How do I detect sample cross-contamination in a batch of SNP array data?

I will be using Axiom Genome-wide Human Origins 1 array. Is there a way to tell whether a sample is contaminated with DNA from another sample during the extraction stage (for example, excessive...

06 March 2020 7,896 3 View

Where is the proper cooling rate measurement position in the transverse-varestraint test(GTWA)?

Maximum crack length and cooling rate measurements are required to calculate the BTR from the transverse-varestraint test. In previous studies, it is stated that the weld pool should be measured...

13 November 2018 9,486 1 View

What happens if broken target used in magnetron sputter?

I deposit SiO2 thin films using magnetron sputter(RF power) and also found the target broke. Buying a new target is unavailable. The RF power sometimes break down when sputtering the...

29 May 2018 6,233 1 View

Is there any possibility of multiple transposon insertions into a chromosome of E. coli?

I learned that a mariner transposon requires only TA dinucleotides for transposition on some DNA strand. (no special sequence prefernce) Let's assume that a cut-and-paste mariner transposon exist...

18 January 2018 8,946 3 View

How can I get clear plant tissue with phloroglucinol staining?

Hi, I am trying to observe lignin through phloroglucinol staining with arabidopsis (petal and sepal). The protocol I am using : 2% phloroglucinol in 100% EtOH , O/N...

31 October 2017 1,975 1 View

SEM preparation for Colepidae (Ciliophora)?

I have a problem for preparing the SEM specimen of Colepidae. The amour plates of the species appears to melt during the preparation procedure. I have attached my preparation and it was prepared...

08 September 2017 6,477 7 View

Do I have to make longer or shorter primer when the PCR is not working

15 August 2017 4,819 24 View

Gel electrophoresis of riboprobes after in vitro transcription supposed to show single band or multiple bands?

Dear all, I am trying to set-up a RNA in situ hybridization experiment. I am reading several protocols for that, and I found one paper says 'if in vitro transcription is performed well, you will...

23 May 2017 4,168 1 View

Is it possible that compressed sensing reconstructed image in MRI maintains or improves SNR comparing to fully sampled original image?

I am not professional about compressed sensing (CS). I know CS can reconstruct the texture of the original image from highly undersampled data if some conditions are met. However, I have no clue...

05 September 2016 2,974 2 View

Can anybody let me know Hansen solubility parameter of Polythiophene?

24 August 2016 1,354 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View