I want to do scoring for macrovesicles and microvesiclular fat diposits in Histopathological Liver sections. Can anyone suggest me a software and discuss a suitable protocol to achieve this. I want to check for the development of Hepatic steatosis or fatty liver condition.
Mehran Hosseini
Dear Anmol Banerjee ,
Previously I used a semiquantitative method for fat accumulation assessment in hepatocytes. the following sentences of our paper (2017) describe the method:" For each section, four to six unbiased counting frames were sampled in a systematic random fashion. Fat accumulation in the liver was graded as previously described (Brunt et al. 1999). Briefly, Grade 0, no fat was found; Grade 1,
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