How to do quantitative estimation of Esterase obtained from bacteria?

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Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

Researchers in the field of AI?

Hi there, this is Dina, a postgraduate student at Warwick University. I am working on my dissertation regarding AI and business. I would like to ask some questions for researchers in this field....

27 June 2024 2,320 2 View

Collaboration needed for LCMS/MS based untargeted metabolomics approach with multivariate analysis of Fermented Rice beverages?

Fermented beverages are very important drinks for some community as they consume daily with huge quantity, are traditionally believed as pharmacological and medicinal property along with...

16 June 2024 7,235 0 View

Is there any relevant calculator to determine household carbon emission in rural Assam?

The existing carbon emission calculator is not suitable for rural households in Assam. As a social scientist, I am searching for such a calculator.

10 June 2024 7,960 0 View

How can I get to the parent molecule name by inputting the MS fragments values?

I did GCMS/MS, LCMS of my sample of interest. I got Mass fragments in ionization process. Now facing issues to get the parent molecules name/formula. Due to complexity of sample (its a mixture of...

01 June 2024 1,056 4 View

Who is/are the latest theorist of classroom management practices?

latest author latest year concept of the theory

10 May 2024 3,518 2 View

Anyone come across by the word "Relational Relativity"?

All my friend in the researchgate, have you heard of the term "Relational Relativity" like the theory of relativity? How can we express in mathematical term like E=MC Square. Can we create a...

08 May 2024 4,952 0 View

Why light and heavy chains from an antibody don’t appear on a SDS-PAGE gel in reducing condition?

Hello, I have purified a monoclonal antibody from an hybridoma supernatant. When I run different eluted fractions on an SDS-PAGE, I get only one band in reducing condition (the 150 kDa band)....

30 April 2024 931 0 View

What is a free software to show the chemical properties of a newly synthesized organic compound?

I have synthesized an organic compound. However, I do not have the access to scifinder or reaxys. I would like to know whether the molecule I have prepared is new or already reported. Is there a...

02 April 2024 901 2 View

Why can't I extract plasmid after storing a cloned plamid in DH5α strain?

Previously I have cloned estA gene in pET28b with restriction sites EcoRI and BamHI and stored it in -80˚C. I also proceeded with site directed mutagenesis (SDM) after confirmation of the cloning...

27 March 2024 8,599 5 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

Hi. Can I get the list of publishers for microbiology-related short communications without publication fee (APC)?

I need to know which journals are exempt from publication fees (APC) in the area of microbiology

11 July 2024 6,346 2 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

What are these aggregates in activated sludge flocs?

I've been working with activated sludge samples and have encountered a challenge in identifying certain structures/organisms that appear abundantly within the flocs during optical microscopy...

23 June 2024 9,584 1 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View