How to do Primary Muller cell isolation from rat retina?

Atiya Saiyed @Atiya-Saiyed

17 October 2024 0 2K Report

Does anybody help me in isolation of muller cells from the rat retina.

Does any one has the protocol for it.

I have seen some paper but not the proper method for that.

Badges
Science topic

More Atiya Saiyed's questions See All

Need help for cq values in Rt-qpcr?

cq values for my reference coming like 22 and cq value of my target gene coming as 23 and 24 around? can i considered this value for future calculation? or what else i can do to get better cq...

14 May 2024 6,081 6 View

How can I prevent Jurkat cell loss after triton-X permeablisation?

Hi, I'm trying to stain for actin in Jurkat cells the staining steps involve: 1. fixing cells in a 96 V bottom plate with 4% PFA 2. centrifuging at 1800 rpm for 3 mins 3. resuspending in 0.1%...

18 February 2024 7,504 0 View

Which publicly available tool will be more suitable to find binding pocket coordinates?

Dear altruist, I have selected a potential enzyme as a drug target in silico. I have also successfully detected the binding pocket of that desired enzyme. Then I tried the ChimeraX tool to find...

01 February 2023 4,393 4 View

How do you assess non-inferiority of survival outcomes when two cancer treatments are compared using matching-adjusted indirect comparison?

Establishing non-inferiority margin in indirect comparison of cancer treatments.

17 August 2022 6,537 1 View

Trial-based cost-effectiveness analysis for advanced cancer where there was significant treatment switching post-progression?

Any reading source for a study that has done a trial-based (individual-patients level data) cost-effectiveness analysis in advanced cancer setting where there was significant treatment switching...

19 November 2019 3,093 0 View

How to do Probabilistic Sensitivity Analysis using gompertz distribution with negative shape parameter in MS Excel for a Partitioned Survival Model?

Health Economics, Cost-effectiveness analysis, Uncertainty analysis and Oncology modelling

26 February 2019 9,003 4 View

QM/MM Setup?

Hi, I am planning to setup QM/MM calculations for my research that involves P-450 complexes and some organic substrates. Please help me in determining which approach to use and possibly a sample...

13 March 2018 3,891 4 View

Handling missing data in EQ-5D-5L responses ?

Hello, I would appreciate if anyone can answer regarding handling missing data in EQ-5D-5L responses? How to decide the method of imputation? Thank you. Best Regards, Masnoon.

03 October 2017 4,991 2 View

Why dna not exit from the well of agarose gel

i have extract dna of bacteriophage and make gel electrophoresis and dna apper in the well only why not exit??

20 October 2016 2,890 5 View

Which data pre_processing technique will be good for applying before Naive Bayes algorithm?

Hello..Please can anyone suggest which data pre_processing technique will be good for applying before Naive Bayes algorithm.Thankyou

14 March 2016 5,323 11 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View