Hi Kurma,

I would recommend to perform IF staining instead of IHC. With IF it is much easier to detect double-positive cells.

With kind regards

Ronald

Hello Kurma,

IF is a good choice when multiple staining is required. You can follow the protocol at:

1. free floating section: http://www.creative-diagnostics.com/immunofluorescence-protocol-free-floating-section.htm

2. Adhesive section: http://www.creative-diagnostics.com/immunofluorescence-protocol-stick-section.htm

Dear Ronald and Langyue,

Thank you very much for your suggestion.