I have a dataset containing the 100 cells' expression value and I have another file where I have the information about which cells are coming from which cluster (clustering was performed before this analysis). Here among 100 cells, 50 cells are from cluster 1 and the other 50 cells are from cluster 2. I have tried to do differential gene expression analysis by DESeq2 and edgeR but was unable to find any differentially expressed genes (It is showing 0 upregulated and 0 downregulated genes ) and the P_adj values are showing 1 for all genes.

It would be very helpful for me if anyone kindly give any hints or insights into this matter.