Questions and seeking cooperation:
1. How to assemble, clean and polish cp genome data from raw data of second generation sequencing? How it can be done in windows system using any platform or software, not in linux.
2. What to do if there are two raw data files of such sequencing data and how to assemble them into one.
3. How to do the consecutive analysis after assemble? what software and platforms to use?
Please do share if any resources available. Moreover, if anyone is interested to collaborate then we will highly appreciate your cooperation. Thanks in advance!
You have asked many questions.
There are different softwares used for Cp genome assembly and analysis but mostly Linux based.
For raw data file you can use reformate.sh which will help you to make one fastq file.
Loubna Youssar Thanks for suggesting this platform. However, our raw data size (fq file) is more than 19GB. So troublesome to deal with analysis. Let me figure out if it can work in Galaxy.
Sure, if I am not wrong, there is no size limitation so far. I mean the galaxy.eu
Set up an account and I may assist if required.
Best
Loubna Youssar Thank you for your reply and your offer to cooperate. let me try and I shall contact you soon with my feedback on what's going on. Let's keep in touch.
Dr. Loubna Youssar , The link above is direct o mars.com! Maybe you mean the site below?
https://usegalaxy.eu/
Dr. Loubna Youssar, Thanks. working on it. Let's see! :)
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