Hello, I did some plasmid sequencing and was provided initially with gz file which I extracted and got FastaQ file. Is there anyway to get fasta sequence from the FastaQ file and also how do we analyze the sequence to know about a gene.
If you have just typed the word "FASTQ" in your browser, the problem would have already been solved.
You can convert FASTQ into FASTA using galaxy web server FASTQ to FASTA tool.
You can use galaxy web server as suggested or online platform: http://sequenceconversion.bugaco.com/converter/biology/sequences/fastq_to_fasta.php
Then just do the blast analysis to know about your gene.
Kiran Dindhoria , I tried using the galaxy web server but size of my file is more than 500 mb hence its out of the limit.
hi you can use the online server http://edwards.sdsu.edu/cgi-bin/prinseq/prinseq.cgi?result=1&i=1333734733 for quality check and file convert FASTq to FASTA
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