How to do agrobacterium plasmid curing?

More Shoeb Quadri's questions See All

Need help to get Alpha EaseFc software to quantitate western blot?

Hello All, I am in need of Alpha EaseFC software to quantitate Immunoblot protein bands. I know other options (such as ImageJ) are available to for this purpose, but I am specifically looking for...

29 May 2023 2,936 1 View

Why VOSviewer (version 1.6.17) shows these marked options for 'Type of Analysis' disabled?

I would like to use VOSviewer for bibliometric analysis and I have used both desktop as well as web versions. However during adding data or creating a map from 'Scopus' or 'Dimensions' on my PC...

07 September 2021 530 3 View

Can someone please share me the list of scopus/sci indexed journals with rapid publication and low publication charges?

Hello, Does anyone have a list of Scopus/SCI-indexed journals with rapid publication and low publication charges in the field of computer security or computer science engineering? Thank You

08 August 2021 3,663 4 View

Can there be a universal COVID-19 vaccine?

There are many strains of Coronavirus causing disease all over the world. It is also mutating. So, is it possible that a single type of vaccine would be effective all over the world? Or should...

14 July 2020 7,106 11 View

Software other than Methylkit for DNA methylation analysis?

I am working on epigenetic modifications with aging, especially DNA methylation. I have .bed, .sam and .bedgraph files obtained in bisulfite DNA sequencing. 1) Is there any software other than...

17 April 2020 4,071 3 View

The roles of bird monitoring in evidence based conservation?

A growing number of researchers and practitioners believe that solutions to prevent further loss and restore global biodiversity (most especially bird species) are about aligning our thinking with...

31 May 2019 9,362 3 View

Why does my sulfur not dissolve in CS2?

Hi, I came across a sample of sulfur powder but it does not dissolve in CS2. What is the possible reason?

01 February 2019 6,310 3 View

What is the importance of viscosity in ICV administration of drug? Can anyone give some reference?

ICV- intracerebroventricular

06 July 2018 7,703 0 View

Anyone has experience with Transscleral cyclophotocoagulation (TSCPC) in glaucoma ?

Micro-pulse TSCPC is becoming popular in glaucoma management as it avoids the complications of glaucoma-filtering-surgeries. However, how effective is this procedure and does it provide any...

01 February 2017 5,757 5 View

Gauss-Siedel based load flow of Droop controlled islanded microgrid?

Hi, I am trying to develop a simple load flow model for droop controlled islanded microgrid using Gauss-Seidel method. It is difficult to have a slack bus for islanded MG. Therefore, it requires...

16 November 2016 4,248 9 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View