How to dissolve partially soluble plant compound and use it for invitro bacterial assays?

Srichandrasekar Indhuprakash @Srichandrasekar-Indhuprakash

30 September 2023 1 3K Report

I have a doubt regarding the plant compound that is partially soluble in water, which I need to test for anti-bacterial activity.

I'm dissolving it in 50% ethanol and using it for anti-bacterial activity tests. Is that fine to use like that?

Can anyone please suggest me some better options?

Thank you in advance

Robert Adolf Brinzer

Very few bacteria do well with total ethanol over 16%. Try using DMSO or a surfactant like Tween-20/80.

Just remember to have a pure ethanol control.

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