11 Questions 21 Answers 0 Followers
Questions related from Srichandrasekar Indhuprakash
I have a doubt regarding the plant compound that is partially soluble in water, which I need to test for anti-bacterial activity. I'm dissolving it in 50% ethanol and using it for anti-bacterial...
30 September 2023 2,825 1 View
Hello all, I hope everyone is doing well. We are preparing buttermilk from standardized milk. In this regard we need to increase the pH of the buttermilk. We used sodium citrate (0.4% &...
29 September 2023 5,559 14 View
Hello all, I have serially diluted mice fecal sample and plated -1, -3, -5, -7 dilutions in the selective medium (E.coli). In -5, -7 dilutions, no growth were seen. But in -3 and -1 dilution, I...
06 May 2022 2,822 1 View
Hello everyone Hope all are doing good. I have a problem with western blot. I'm using Biorad set up for the whole process. I did it 5 times, in the initial stage the protein transfer was not...
30 December 2021 7,774 8 View
Dear all, Hope everyone fine. I'm having a doubt regarding that I'm going to do several in-vitro assays in subsequent days with the bacterial cells. In this regard, Shall I Culture a particular...
16 April 2021 5,288 11 View
Hello everyone, I have collected a fecal sample and dissolved it in 1mL of TSB broth. Then I have serially diluted the sample to 9 dilutions. In this condition, I have taken the 5th dilution, 7th...
02 November 2020 4,362 10 View
Hello everyone, Hope all are fine. I have a doubt about SDS-PAGE. I have done 4 gels (details were given in the attachment file) for the same known targeted protein contains two bands. In all the...
23 October 2020 4,791 7 View
Hello all, I'm in the need of Escherichia coli strain which causes diarrhea in humans. Is anyone having this diarrhea causing organism or Can you prefer me if you have undergone this specific...
17 September 2020 3,842 1 View
Hello everyone, Hope all are safe. I have done SDS-PAGE for my desired protein from a microbial source and immunoglobulin. In this regard, I have loaded 100 microgram protein in each well, but...
09 September 2020 791 15 View
Hello everyone, hoping that all are safe. I have a doubt that "Is there any coloring agent or dye to make a colorless bacterial whole-cell antigen to colored one?" Kindly provide your valuable...
24 August 2020 8,768 0 View
Hello everyone, Hope all are fine and safe. I have a doubt about the nitrocellulose membrane for western blot analysis. Is there any specific pore size in the nitrocellulose membrane for the...
01 January 1970 4,776 6 View
